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- EC HPLC column (analytical), NUCLEOSHELL HILIC, 2.7 µm, 150x4 mm
Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on core-shell technology silica gel particles.
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Particle shape
Spherical
Endcapped
No
Pore size
90 Å
USP listing
Not available or proprietary
Recommended application(s)
Amino acids
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Pressure stability (max)
600 bar
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
4 mm
Column length
150 mm
Column volume
1.9 mL
Recommended application(s)
Water-soluble vitamins
Recommended application(s)
Peptides
Recommended application(s)
HILIC applications HPLC
Recommended application(s)
Highly polar analytes
Recommended application(s)
Hydrophilic compounds
Recommended application(s)
Organic polar acids and bases
Recommended application(s)
Polar natural compounds
Recommended application(s)
Nucleosides
Recommended application(s)
Oligonucleotides
pH stability
2.0–8.5
Mode
Hydrophilic Interaction Chromatography (HILIC)
Surface chemistry
Ammonium – sulfonic acid (zwitterionic) modification
Brand
NUCLEOSHELL
Pore volume
Not available or proprietary
Base material
NUCLEOSHELL - core-shell silica
Particle type
Core-shell, superficially porous particles (SPP)
Particle size
2.7 µm
Specific surface according to BET
130 m²/g
Phase
NUCLEOSHELL HILIC
Carbon content
1.3 %
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