SLE/LLE – Extraction without formation of emulsions

Liquid‑liquid extraction (LLE) or solid liquid extraction (SLE) of highly viscous aqueous solutions e.g., physiological fluids (blood, plasma, serum), dyes in textiles etc. can be performend with CHROMABOND XTR. Our product enables you to simultaneous preparation of several samples, fast, reproducible and economical.


Kieselguhr phase for liquid-liquid extraction (LLE), solid or supported liquid extraction (SLE)

The base material of CHROMABOND XTR coarse-grained kieselguhr (also known as diatomaceous earth, hydromatrix, celite) provides a large pore size, high pore volume, constantly high batch-to-batch quality. It can be used in the pH working range of 1–13.

Depending on the concentration of the analytes eluates can be analyzed immediately, or the organic solvent is evaporated. The pH value of the aqueous solution can be altered on the column, which enables elution of different compounds of a sample under optimized conditions. Under certain circumstances, acidic, neutral, and basic compounds can be fractionated in this way. Eluents with too high alcohol contents cause an increase in volume of the aqueous phase on the CHROMABOND XTR. Here the column could be overloaded and the aqueous phase displaced from the column. In this case, a greater capacity column should be used.

Solvents applicable for elution:
  • Diethyl ether
  • tert-butyl methyl ether
  • Ethyl acetate
  • n-hexane
  • Cyclohexane
  • Toluene
  • Dichloromethane (methylene chloride)
  • Trichloromethane (chloroform)

Solvent mixtures:

  • Trichloromethane – methanol (90:10, v/v)
  • Trichloromethane – methanol (85:15, v/v)
  • Diethyl ether – ethanol (90:10, v/v)
  • Diethyl ether – ethanol (80:20, v/v)
  • Dichloromethane – 2-propanol (90:10, v/v)
  • Dichloromethane – 2-propanol (85:15, v/v)


  • Liquid-liquid extraction of highly viscous aqueous solutions such as physiological fluids (blood, plasma, and serum) in clinical chemistry, dyes in textiles, environmental and food analysis without use of a separation funnel
  • High water loadability without breakthrough of water during elution with organic solvents
  • Also suited for removing small amounts of water from solvents which are not miscible with water


  • Fast, reproducible and economical
  • Simultaneous preparation of several samples
  • No problems with phase separation ∙ no formation of emulsions
  • High recovery rates
  • Saving of time and solvents
  • Organic solutions need not to be dried after separation