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- VA HPLC column (analytical), NUCLEOGEL SUGAR Ca, 8-10 µm, 300x6.5 mm
Polymer-based HPLC phase for separation of sugars. Sulfonated polystyrene / divinylbenzene resins in Ca ionic form. Separation mechanism includes steric exclusion, ligand exchange and partition effects, ligand exchange being the predominant force, since the hydrated metal ions form strong interactions with the hydroxyl groups of the sample molecules. The intensity of these interactions decreases in the sequence Pb, Ca, Na.
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Recommended application(s)
HPLC
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
Mode
Special application or diverse
Pore size
100 Å
Recommended application(s)
Sugars
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Method
HPLC, analytical
Hardware
Stainless steel
Carbon content
Not available or proprietary
Brand
NUCLEOGEL
Recommended application(s)
Mono- and oligosaccharides
Recommended application(s)
Sugar alcohols
Temperature stability (max)
90 °C
Column length
300 mm
Pressure stability (max)
70 bar
Type
VA HPLC column (analytical)
Base material
Sulfonated spherical PS/DVB resin
Eluent in HPLC column
Demineralized water + 0.02 % NaN₃ (sodium azide)
Column inner diameter
6.5 mm
Column volume
9.95 mL
USP listing
L19
Phase
NUCLEOGEL SUGAR Ca
Particle size
8–10 µm
Surface chemistry
Sulfonated spherical polystyrene resin cross-linked with divinylbenzene (PS/DVB), Ca²⁺ form
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