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- VP HPLC column (preparative), NUCLEOGEN 500-7 DEAE, 7 µm, 125x10 mm
Silica-based HPLC phase for anion exchange chromatography of nucleic acids. For the separation of tRNA, 5S RNA, viroids and messenger RNA in the intermediate molecular weight range (25,000–1,000,000 daltons) with recoveries > 95 %. Capacity 730 A260 for a 125 x 6 mm ID column, 1940 A260 for a 125 x 10 mm ID column.
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Recommended application(s)
HPLC
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
Base material
Silica gel
Mode
Special application or diverse
Mode
Ion exchange (IEX)
Particle size
7 µm
USP listing
Not available or proprietary
Hardware
Stainless steel
Column length
125 mm
Carbon content
Not available or proprietary
Pore volume
Not available or proprietary
Specific surface according to BET
Not available or proprietary
Pressure stability (max)
250 bar
Pore size
500 Å
Brand
NUCLEOGEN
Temperature stability (max)
70 °C
pH stability
2.5–8.0
Surface chemistry
Silica gel based anion exchanger with hydrophilic coating
Eluent in HPLC column
Methanol
Scope of delivery
1x semi-preparative HPLC column, instruction leaflet, certificate of analysis
Column inner diameter
10 mm
Method
HPLC, preparative
Type
VP HPLC column (preparative)
Recommended application(s)
tRNA
Recommended application(s)
5S RNA
Recommended application(s)
Viroids
Recommended application(s)
Messenger RNA
Recommended application(s)
Intermediate molecular weight range (25,000–1,000,000 daltons)
Phase
NUCLEOGEN 500-7 DEAE
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