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- VP HPLC column (preparative), NUCLEODUR C8 ec, 5 µm, 250x21 mm
Standard RP phase for daily routine analysis and up-scaling for preparative HPLC. This octyl modification is based on high purity NUCLEODUR silica gel. C8 has a lower retention of hydrophobic compounds compared to C18 (octadecyl silane, ODS).
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Brand
NUCLEODUR
Pore size
110 Å
Specific surface according to BET
340 m²/g
Base material
NUCLEODUR - high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Mode
Reversed phase (RP)
Endcapped
Yes
Particle size
5 µm
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Hardware
Stainless steel
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column length
250 mm
pH stability
1.0–9.0
USP listing
L7
Surface chemistry
Medium density monomeric octyl (C8, MOS) modification
Pressure stability (max)
400 bar
Method
HPLC, preparative
Type
VP HPLC column (preparative)
Scope of delivery
1x preparative HPLC column, instruction leaflet, certificate of analysis
Recommended application(s)
RP applications (preparative)
Column inner diameter
21 mm
Recommended flow rate
14–40 mL/min
Recommended application(s)
For examples please see analytical NUCLEODUR C8 ec
Phase
NUCLEODUR C8 ec
Column volume
87 mL
Carbon content
10.5%
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