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- VP HPLC column (preparative), NUCLEODUR PolarTec, 5 µm, 250x21 mm
Based on high-purity NUCLEODUR silica gel, a polar group was embedded in a hydrophobic C18 modification. As a result, this HPLC phase shows steric and an increased polar selectivity. This HPLC phase shows a good performance under high aqueous conditions. It is a hydrophobic phase with pronounced hydrophilic properties. Separation mechanism based on hydrophobic (van der Waals) and polar interactions. Suitable for LC/MS due to low bleeding characteristics.
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Brand
NUCLEODUR
Pore size
110 Å
Specific surface according to BET
340 m²/g
Base material
NUCLEODUR - high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Mode
Reversed phase (RP)
USP listing
L1
Endcapped
Yes
Particle size
5 µm
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Hardware
Stainless steel
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column length
250 mm
pH stability
1.0–9.0
Carbon content
17.0 %
USP listing
L60
Phase
NUCLEODUR PolarTec
Pressure stability (max)
400 bar
Method
HPLC, preparative
Type
VP HPLC column (preparative)
Scope of delivery
1x preparative HPLC column, instruction leaflet, certificate of analysis
Recommended application(s)
RP applications (preparative)
Column inner diameter
21 mm
Recommended flow rate
14–40 mL/min
Recommended application(s)
For examples please see analytical NUCLEODUR PolarTec
Surface chemistry
Octadecyl phase (C18, ODS) modification, with embedded polar group (PEG)
Column volume
87 mL
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