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- EC HPLC column (analytical), NUCLEOSHELL HILIC, 2.7 µm, 100x3 mm
Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on core-shell technology silica gel particles.
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			Particle shape Spherical Endcapped No Pore size 90 Å USP listing Not available or proprietary Recommended application(s) Amino acids Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Temperature stability (max) 60 °C Tmax (phosphate buffer) 40 °C Pressure stability (max) 600 bar Method HPLC, analytical Hardware Stainless steel Type EC HPLC column (analytical) Eluent in HPLC column Acetonitrile – water Column inner diameter 3 mm Column length 100 mm Column volume 0.7 mL Recommended application(s) Water-soluble vitamins Recommended application(s) Peptides Recommended application(s) HILIC applications HPLC Recommended application(s) Highly polar analytes Recommended application(s) Hydrophilic compounds Recommended application(s) Organic polar acids and bases Recommended application(s) Polar natural compounds Recommended application(s) Nucleosides Recommended application(s) Oligonucleotides pH stability 2.0–8.5 Mode Hydrophilic Interaction Chromatography (HILIC) Surface chemistry Ammonium – sulfonic acid (zwitterionic) modification Brand NUCLEOSHELL Pore volume Not available or proprietary Base material NUCLEOSHELL - core-shell silica Particle type Core-shell, superficially porous particles (SPP) Particle size 2.7 µm Specific surface according to BET 130 m²/g Phase NUCLEOSHELL HILIC Carbon content 1.3 % 
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