EC HPLC column (analytical), NUCLEOSIL 120-3 C18, 3 µm, 250x4 mm
 

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Macherey Nagel - Chromatography / HPLC column (analytical)

EC HPLC column (analytical), NUCLEOSIL 120-3 C18, 3 µm, 250x4 mm

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SKU: 720055.4

EC HPLC column (analytical), NUCLEOSIL 120-3 C18, 3 µm, 250x4 mm

This HPLC column is packed with endcapped C18 (ODS, octadecyl silyl) modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

  • Particle type

    Fully porous particles (FPP)

    Particle shape

    Spherical

    pH stability

    2.0–8.0

    Mode

    Reversed phase (RP)

    USP listing

    L1

    Endcapped

    Yes

    Surface chemistry

    Octadecyl (C18, ODS) modification

    Recommended application(s)

    Pesticides

    Scope of delivery

    1x analytical HPLC column, instruction leaflet, certificate of analysis

    Recommended application(s)

    RP applications HPLC

    Recommended application(s)

    Reversed phase

    Temperature stability (max)

    60 °C

    Tmax (phosphate buffer)

    40 °C

    Method

    HPLC, analytical

    Hardware

    Stainless steel

    Type

    EC HPLC column (analytical)

    Particle size

    3 µm

    Eluent in HPLC column

    Acetonitrile – water

    Column inner diameter

    4 mm

    Column length

    250 mm

    Column volume

    3.15 mL

    Carbon content

    11.0 %

    Recommended application(s)

    Fat-soluble vitamins

    Recommended application(s)

    Derivatized amino acids

    Recommended application(s)

    Aldehydes

    Recommended application(s)

    Ketones

    Recommended application(s)

    Phenolic compounds

    Recommended application(s)

    Basic, neutral or acidic drugs

    Brand

    NUCLEOSIL

    Pressure stability (max)

    400 bar

    Base material

    NUCLEOSIL - the standard silica

    Pore size

    120 Å

    Pore volume

    0.65 mL/g

    Specific surface according to BET

    200 m²/g

    Phase

    NUCLEOSIL 120-3 C18

  • Macherey Nagel - Chromatography Catalogue