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- EC HPLC column (analytical), NUCLEOSIL 300-5 C18, 5 µm, 250x4 mm
This HPLC column is packed with endcapped C18 (ODS, octadecyl silyl) modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.
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Particle type
Fully porous particles (FPP)
Particle shape
Spherical
pH stability
2.0–8.0
Mode
Reversed phase (RP)
USP listing
L1
Endcapped
Yes
Surface chemistry
Octadecyl (C18, ODS) modification
Particle size
5 µm
Pore size
300 Å
Recommended application(s)
Pesticides
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Recommended application(s)
RP applications HPLC
Recommended application(s)
Reversed phase
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
4 mm
Column length
250 mm
Column volume
3.15 mL
Recommended application(s)
Fat-soluble vitamins
Recommended application(s)
Derivatized amino acids
Recommended application(s)
Aldehydes
Recommended application(s)
Ketones
Recommended application(s)
Phenolic compounds
Recommended application(s)
Basic, neutral or acidic drugs
Specific surface according to BET
100 m²/g
Brand
NUCLEOSIL
Base material
NUCLEOSIL - the standard silica
Carbon content
6.5 %
Pore volume
0.8 mL/g
Pressure stability (max)
300 bar
Phase
NUCLEOSIL 300-5 C18
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