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- EC HPLC column (analytical), NUCLEOSIL CHIRAL-1, 5 µm, 250x4 mm
Silica-based phase for HPLC enantiomer separation. Principal interaction mode - formation of ternary mixed-ligand complexes with Cu(II) ions. Differences in the stability of the diastereomeric complexes cause chromatographic separation. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.
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Recommended application(s)
HPLC
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
pH stability
2.0–8.0
Mode
Special application or diverse
Particle size
5 µm
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Temperature stability (max)
60 °C
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Column inner diameter
4 mm
Column length
250 mm
Column volume
3.15 mL
Carbon content
Not available or proprietary
Brand
NUCLEOSIL
Pressure stability (max)
400 bar
Base material
NUCLEOSIL - the standard silica
Pore size
120 Å
Pore volume
0.65 mL/g
Recommended application(s)
Chiral, enantiomers
Recommended application(s)
Enantiomers with two polar functional groups
Recommended application(s)
α-amino acids
Recommended application(s)
α-hydroxycarboxylic acids
Recommended application(s)
Lactic acid
USP listing
L32
Phase
NUCLEOSIL CHIRAL-1
Specific surface according to BET
200 m²/g
Surface chemistry
L-hydroxyproline – Cu²⁺ complexes (chiral selector) modification
Eluent in HPLC column
0.5 mmol/L copper sulfate solution
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