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- EC HPLC column (analytical), NUCLEOSIL 50-5, 5 µm, 250x4.6 mm
This HPLC column is packed with unmodified original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.
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Recommended application(s)
HPLC
Mode
Normal phase (NP)
USP listing
L3
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Surface chemistry
Unmodified silica gel (SiOH)
Endcapped
No
pH stability
2.0–8.0
Particle size
5 µm
Pore size
50 Å
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Column inner diameter
4.6 mm
Column length
250 mm
Column volume
4.15 mL
Recommended application(s)
Normal phase
Recommended application(s)
NP applications
Eluent in HPLC column
n-heptane
Recommended application(s)
Polar and midpolar compounds
Carbon content
Not available or proprietary
Brand
NUCLEOSIL
Pressure stability (max)
400 bar
Base material
NUCLEOSIL - the standard silica
Pore volume
0.8 mL/g
Phase
NUCLEOSIL 50-5
Specific surface according to BET
420 m²/g
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