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- EC HPLC column (analytical), NUCLEOSIL 120-5 C4, 5 µm, 250x4.6 mm
This HPLC column is packed with butyl modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured exclusively in Germany and is still one of the most used HPLC silicas in quality control around the globe.
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Particle type
Fully porous particles (FPP)
Particle shape
Spherical
pH stability
2.0–8.0
Mode
Reversed phase (RP)
Endcapped
Yes
Particle size
5 µm
Carbon content
4.0 %
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Recommended application(s)
RP applications HPLC
Recommended application(s)
Reversed phase
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
4.6 mm
Column length
250 mm
Column volume
4.15 mL
USP listing
L26
Brand
NUCLEOSIL
Pressure stability (max)
400 bar
Base material
NUCLEOSIL - the standard silica
Pore size
120 Å
Pore volume
0.65 mL/g
Specific surface according to BET
200 m²/g
Phase
NUCLEOSIL 120-5 C4
Surface chemistry
Medium density monomeric butyl (C4) modification
Recommended application(s)
Ion-pairing chromatography
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