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- EC HPLC column (analytical), NUCLEOSIL 100-5 Protect I, 5 µm, 125x4 mm
RP phase with pronounced hydrophilic properties. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.
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Particle type
Fully porous particles (FPP)
Particle shape
Spherical
pH stability
2.0–8.0
Mode
Reversed phase (RP)
Endcapped
Yes
Pore size
100 Å
Particle size
5 µm
Recommended application(s)
Pesticides
USP listing
Not available or proprietary
Recommended application(s)
Amino acids
Recommended application(s)
Organic acids
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Recommended application(s)
RP applications HPLC
Recommended application(s)
Reversed phase
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
4 mm
Column length
125 mm
Column volume
1.56 mL
Recommended application(s)
Water-soluble vitamins
Carbon content
11.0 %
Recommended application(s)
Polar compounds
Recommended application(s)
Basic pharmaceuticals
Brand
NUCLEOSIL
Pressure stability (max)
400 bar
Pore volume
1 mL/g
Specific surface according to BET
350 m²/g
Base material
NUCLEOSIL - the standard silica
Recommended application(s)
Phenols and nitrogen containing compounds
Phase
NUCLEOSIL 100-5 Protect I
Surface chemistry
Protective polar group, monomeric modification
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