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- EC HPLC column (analytical), NUCLEOSIL 100-5 SB, 5 µm, 150x4.6 mm
Strongly basic anion exchanger (SAX) with capacity ~ 1 meq/g. Separation mechanism based on strong ion exchange interactions. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.
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Recommended application(s)
HPLC
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
pH stability
2.0–8.0
Mode
Ion exchange (IEX)
Pore size
100 Å
Particle size
5 µm
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Column inner diameter
4.6 mm
Column length
150 mm
Column volume
2.5 mL
Carbon content
10.0 %
Brand
NUCLEOSIL
Pressure stability (max)
400 bar
Pore volume
1 mL/g
Specific surface according to BET
350 m²/g
Base material
NUCLEOSIL - the standard silica
Eluent in HPLC column
0.15 mol/L (NH₄)₂HPO₄ (diammonium hydrogen phosphate), pH 5
Recommended application(s)
Ion exchange
Recommended application(s)
Nucleic acids
Recommended application(s)
Uronic acids
Recommended application(s)
Oligogalacturonic acids
Recommended application(s)
Inorganic anions
Recommended application(s)
Hydrogen carbonate
Recommended application(s)
Hydrogen phosphate
Recommended application(s)
Chloride
Recommended application(s)
Nitrite
Recommended application(s)
Bromide
Recommended application(s)
Nitrate
Recommended application(s)
Sulfate
Recommended application(s)
Iodide
Recommended application(s)
Catecholamines
Recommended application(s)
L-glutamine
Recommended application(s)
Inorganic and organic ionic species
Recommended application(s)
Bialaphos
Recommended application(s)
Glufosinate
Recommended application(s)
Glyphosate
Recommended application(s)
Phosphorus-containing amino acid
Recommended application(s)
Herbicide
USP listing
L14
Surface chemistry
Quaternary ammonium (strong basic anion exchanger - SAX) modification
Phase
NUCLEOSIL 100-5 SB
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