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- EC HPLC column (analytical), NUCLEOSIL 100-3 C18 HD, 3 µm, 125x3 mm
This HPLC column is packed with high density (HD and endcapped) C18 (ODS, octadecyl silyl) modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.
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			Particle shape Spherical Mode Reversed phase (RP) USP listing L1 Endcapped Yes Pore size 100 Å Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Recommended application(s) RP applications HPLC Recommended application(s) Reversed phase Recommended application(s) Pharmaceuticals Recommended application(s) Analgesics Recommended application(s) Antiinflammatory drugs Recommended application(s) Antidepressants Recommended application(s) Herbicides Recommended application(s) Phytopharmaceuticals Recommended application(s) Immunosuppressants Temperature stability (max) 60 °C Tmax (phosphate buffer) 40 °C Method HPLC, analytical Hardware Stainless steel Type EC HPLC column (analytical) Particle size 3 µm Surface chemistry High density octadecyl (C18, ODS) modification Eluent in HPLC column Acetonitrile – water Column inner diameter 3 mm Column length 125 mm Column volume 0.87 mL Carbon content 20.0 % Brand NUCLEOSIL Pressure stability (max) 400 bar pH stability 2.0–9.0 Phase NUCLEOSIL 100-3 C18 HD Pore volume 1 mL/g Specific surface according to BET 350 m²/g Base material NUCLEOSIL - the standard silica Particle type Fully porous particle (FPP) 
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