EC HPLC column (analytical), NUCLEOSIL 100-5 C18 AB, 5 µm, 250x4 mm
 

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Macherey Nagel - Chromatography / HPLC column (analytical)

EC HPLC column (analytical), NUCLEOSIL 100-5 C18 AB, 5 µm, 250x4 mm

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SKU: 720936.4

EC HPLC column (analytical), NUCLEOSIL 100-5 C18 AB, 5 µm, 250x4 mm

Endcapped RP phase with crosslinked octadecyl modification with high steric selectivity. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

  • Particle type

    Fully porous particles (FPP)

    Particle shape

    Spherical

    Mode

    Reversed phase (RP)

    USP listing

    L1

    Endcapped

    Yes

    Pore size

    100 Å

    Particle size

    5 µm

    Recommended application(s)

    Steroids

    Scope of delivery

    1x analytical HPLC column, instruction leaflet, certificate of analysis

    Recommended application(s)

    RP applications HPLC

    Recommended application(s)

    Reversed phase

    Temperature stability (max)

    60 °C

    Tmax (phosphate buffer)

    40 °C

    Method

    HPLC, analytical

    Hardware

    Stainless steel

    Type

    EC HPLC column (analytical)

    Eluent in HPLC column

    Acetonitrile – water

    Column inner diameter

    4 mm

    Column length

    250 mm

    Column volume

    3.15 mL

    pH stability

    1.0–9.0

    Recommended application(s)

    (o,p,m-) Substituted aromatics

    Recommended application(s)

    Fat-soluble vitamins

    Surface chemistry

    Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification

    Surface chemistry

    High steric selectivity

    Brand

    NUCLEOSIL

    Pressure stability (max)

    400 bar

    Pore volume

    1 mL/g

    Specific surface according to BET

    350 m²/g

    Base material

    NUCLEOSIL - the standard silica

    Phase

    NUCLEOSIL 100-5 C18 AB

    Carbon content

    25.0 %

  • Macherey Nagel - Chromatography Catalogue