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- EC HPLC column (analytical), NUCLEOSIL 100-5 C18 AB, 5 µm, 50x3 mm
Endcapped RP phase with crosslinked octadecyl modification with high steric selectivity. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.
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Particle shape
Spherical
Mode
Reversed phase (RP)
USP listing
L1
Endcapped
Yes
Pore size
100 Å
Particle size
5 µm
Recommended application(s)
Steroids
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Recommended application(s)
RP applications HPLC
Recommended application(s)
Reversed phase
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Column length
50 mm
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
3 mm
Column volume
0.35 mL
pH stability
1.0–9.0
Recommended application(s)
(o,p,m-) Substituted aromatics
Recommended application(s)
Fat-soluble vitamins
Surface chemistry
Nonpolar crosslinked polymeric octadecyl (C18, ODS) modification
Surface chemistry
High steric selectivity
Brand
NUCLEOSIL
Pressure stability (max)
400 bar
Pore volume
1 mL/g
Specific surface according to BET
350 m²/g
Base material
NUCLEOSIL - the standard silica
Phase
NUCLEOSIL 100-5 C18 AB
Carbon content
25.0 %
Particle type
Fully porous particle (FPP)
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