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For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.
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Recommended application(s)
HPLC
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
Mode
Special application or diverse
Pore size
100 Å
Particle size
5 µm
USP listing
Not available or proprietary
Hardware
Stainless steel
Column inner diameter
3 mm
Carbon content
Not available or proprietary
Pressure stability (max)
400 bar
Pore volume
1 mL/g
Specific surface according to BET
350 m²/g
Base material
NUCLEOSIL - the standard silica
Brand
NUCLEODEX
Temperature stability (max)
50 °C
pH stability
3.0–8.0
Phase
NUCLEODEX ALPHA-PM
Surface chemistry
Permethylated α-cyclodextrin (chiral selector) modification
Eluent in HPLC column
CH₃OH (methanol) – 50 mmol/L phosphate, pH 3 (70/30, v/v)
Recommended application(s)
Guard column, ideal protection for your main column
Method
HPLC, protection
Column length
4 mm
Type
EC HPLC guard column (analytical)
Scope of delivery
1x analytical HPLC guard column
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