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Silica-based HPLC phase for anion exchange chromatography of nucleic acids. For separation of oligonucleotides up to chain lengths of 40 bases with recoveries > 95 %. Capacity 200 A260/mL (~ 300 A260 for a 125 x 4 mm ID column, 1875 A260 for a 125 x 10 mm ID column). Preparative separations possible when using higher flow rates and longer gradient times.
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Recommended application(s)
HPLC
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
Pore size
60 Å
Base material
Silica gel
Mode
Special application or diverse
Mode
Ion exchange (IEX)
Particle size
7 µm
USP listing
Not available or proprietary
Hardware
Stainless steel
Column length
125 mm
Carbon content
Not available or proprietary
Pore volume
Not available or proprietary
Specific surface according to BET
Not available or proprietary
Pressure stability (max)
250 bar
Brand
NUCLEOGEN
Recommended application(s)
Oligonucleotides up to chain lengths of 40 bases
Temperature stability (max)
70 °C
pH stability
2.5–8.0
Phase
NUCLEOGEN 60-7 DEAE
Surface chemistry
Silica gel based anion exchanger with hydrophilic coating
Eluent in HPLC column
Methanol
Scope of delivery
1x semi-preparative HPLC column, instruction leaflet, certificate of analysis
Column inner diameter
10 mm
Method
HPLC, preparative
Type
VP HPLC column (preparative)
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