VA HPLC column (analytical), NUCLEOGEN 500-7 DEAE, 7 µm, 125x6 mm
 

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Macherey Nagel - Chromatography / HPLC column (analytical)

VA HPLC column (analytical), NUCLEOGEN 500-7 DEAE, 7 µm, 125x6 mm

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SKU: 736598

VA HPLC column (analytical), NUCLEOGEN 500-7 DEAE, 7 µm, 125x6 mm

Silica-based HPLC phase for anion exchange chromatography of nucleic acids. For the separation of tRNA, 5S RNA, viroids and messenger RNA in the intermediate molecular weight range (25,000–1,000,000 daltons) with recoveries > 95 %. Capacity 730 A260 for a 125 x 6 mm ID column, 1940 A260 for a 125 x 10 mm ID column.

  • Recommended application(s)

    HPLC

    Particle type

    Fully porous particles (FPP)

    Particle shape

    Spherical

    Endcapped

    No

    Base material

    Silica gel

    Mode

    Special application or diverse

    Mode

    Ion exchange (IEX)

    Particle size

    7 µm

    USP listing

    Not available or proprietary

    Scope of delivery

    1x analytical HPLC column, instruction leaflet, certificate of analysis

    Method

    HPLC, analytical

    Hardware

    Stainless steel

    Column length

    125 mm

    Carbon content

    Not available or proprietary

    Pore volume

    Not available or proprietary

    Specific surface according to BET

    Not available or proprietary

    Type

    VA HPLC column (analytical)

    Pressure stability (max)

    250 bar

    Pore size

    500 Å

    Brand

    NUCLEOGEN

    Temperature stability (max)

    70 °C

    pH stability

    2.5–8.0

    Surface chemistry

    Silica gel based anion exchanger with hydrophilic coating

    Eluent in HPLC column

    Methanol

    Recommended application(s)

    tRNA

    Recommended application(s)

    5S RNA

    Recommended application(s)

    Viroids

    Recommended application(s)

    Messenger RNA

    Recommended application(s)

    Intermediate molecular weight range (25,000–1,000,000 daltons)

    Column inner diameter

    6 mm

    Phase

    NUCLEOGEN 500-7 DEAE

  • Macherey Nagel - Chromatography Catalogue