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- VP HPLC column (preparative), NUCLEOGEN 4000-7 DEAE, 7 µm, 125x10 mm
Silica-based HPLC phase for anion exchange chromatography of nucleic acids. For the separation of plasmids, DNA restriction fragments, ribosomal RNA, messenger RNA and viral RNA, i.e., very high molecular weight nucleic acids (e.g., 1–50 megadaltons). Capacity 120 A260 for a 125 x 6 mm ID column, 350 A260 for a 125 x 10 mm ID column.
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Recommended application(s)
HPLC
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
Base material
Silica gel
Mode
Special application or diverse
Mode
Ion exchange (IEX)
Particle size
7 µm
USP listing
Not available or proprietary
Hardware
Stainless steel
Column length
125 mm
Carbon content
Not available or proprietary
Pore volume
Not available or proprietary
Specific surface according to BET
Not available or proprietary
Pressure stability (max)
250 bar
Brand
NUCLEOGEN
Temperature stability (max)
70 °C
pH stability
2.5–8.0
Surface chemistry
Silica gel based anion exchanger with hydrophilic coating
Eluent in HPLC column
Methanol
Scope of delivery
1x semi-preparative HPLC column, instruction leaflet, certificate of analysis
Column inner diameter
10 mm
Method
HPLC, preparative
Type
VP HPLC column (preparative)
Recommended application(s)
Messenger RNA
Recommended application(s)
Plasmids
Recommended application(s)
DNA restriction fragments
Recommended application(s)
Ribosomal RNA
Recommended application(s)
Viral RNA
Recommended application(s)
Very high molecular weight nucleic acids (e.g., 1–50 megadaltons)
Phase
NUCLEOGEN 4000-7 DEAE
Pore size
4000 Å
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