EC HPLC column (analytical), NUCLEODUR C18 Gravity, 5 µm, 125x4.6 mm
 

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Macherey Nagel - Chromatography / HPLC column (analytical)

EC HPLC column (analytical), NUCLEODUR C18 Gravity, 5 µm, 125x4.6 mm

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SKU: 760100.46

EC HPLC column (analytical), NUCLEODUR C18 Gravity, 5 µm, 125x4.6 mm

This high density C18 phase based on high purity NUCLEODUR silica gel with its endcapping suppresses any unwanted polar interactions between the silica surface and the sample, which makes “Gravity” particularly suitable for the separation of basic and other ionizable analytes. Due to the enhanced pH stability, LC/MS suitability and superior base deactivation (BDS) it is a perfect choice for method development and valuable for RP applications.

  • Brand

    NUCLEODUR

    Pore size

    110 Å

    Specific surface according to BET

    340 m²/g

    Base material

    NUCLEODUR - high purity silica

    Particle type

    Fully porous particles (FPP)

    Particle shape

    Spherical

    Mode

    Reversed phase (RP)

    USP listing

    L1

    Endcapped

    Yes

    Particle size

    5 µm

    pH stability

    1.0–11.0

    Scope of delivery

    1x analytical HPLC column, instruction leaflet, certificate of analysis

    Recommended application(s)

    RP applications HPLC

    Recommended application(s)

    Reversed phase

    Recommended application(s)

    Pharmaceuticals

    Recommended application(s)

    Analgesics

    Recommended application(s)

    Antiinflammatory drugs

    Recommended application(s)

    Antidepressants

    Recommended application(s)

    Herbicides

    Recommended application(s)

    Phytopharmaceuticals

    Recommended application(s)

    Immunosuppressants

    Recommended application(s)

    Applications at pH extremes (1-11)

    Recommended application(s)

    Ideal for method development

    Temperature stability (max)

    60 °C

    Tmax (phosphate buffer)

    40 °C

    Pressure stability (max)

    600 bar

    Method

    HPLC, analytical

    Hardware

    Stainless steel

    Type

    EC HPLC column (analytical)

    Phase

    NUCLEODUR C18 Gravity

    Pore volume

    0.9 mL/g

    Carbon content

    18.0 %

    Surface chemistry

    High density octadecyl (C18, ODS) modification

    Surface chemistry

    Base deactivated (BDS)

    Eluent in HPLC column

    Acetonitrile – water

    Column inner diameter

    4.6 mm

    Column length

    125 mm

    Column volume

    2.06 mL

  • Macherey Nagel - Chromatography Catalogue