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- EC HPLC column (analytical), NUCLEODUR 300-5 C18 ec, 5 µm, 150x3 mm
Standard RP phase with wide pores (300 Å) for separation of biomolecules in analytical columns. This octadecyl modification is based on high purity NUCLEODUR silica gel.
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Brand
NUCLEODUR
Base material
NUCLEODUR - high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Mode
Reversed phase (RP)
USP listing
L1
Endcapped
Yes
Particle size
5 µm
Pore size
300 Å
Carbon content
4.0 %
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Recommended application(s)
RP applications HPLC
Recommended application(s)
Reversed phase
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
3 mm
Column length
150 mm
Column volume
1.05 mL
pH stability
1.0–9.0
Pressure stability (max)
450 bar
Specific surface according to BET
100 m²/g
Surface chemistry
Medium density monomeric octadecyl (C18, ODS) wide-pore modification
Phase
NUCLEODUR 300-5 C18 ec
Recommended application(s)
300 Å phase for proteins and peptides
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