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- EC UHPLC column (analytical), NUCLEODUR PFP, 1.8 µm, 100x4.6 mm
This hydrophobic phase with pentafluorophenyl-propyl modification is based on high purity NUCLEODUR silica gel and gives an alternative selectivity in comparison to classical C18 modifications. Separation mechanism based on four retention mechanisms - polar interactions (H bonds), dipole-dipole interactions, π-π interactions and hydrophobic interactions. Suitable for LC/MS due to low bleeding characteristics.
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Brand
NUCLEODUR
Pore size
110 Å
Specific surface according to BET
340 m²/g
Base material
NUCLEODUR - high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Mode
Reversed phase (RP)
Endcapped
Yes
Recommended application(s)
Antibiotics
Recommended application(s)
Phenols
Recommended application(s)
Pharmaceuticals
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Pressure stability (max)
600 bar
Hardware
Stainless steel
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
4.6 mm
Column length
100 mm
Column volume
1.65 mL
pH stability
1.0–9.0
Recommended application(s)
Suitable for LC-MS
Recommended application(s)
Polar compounds
Recommended application(s)
RP applications UHPLC
Recommended application(s)
Aromatic and unsaturated compounds
Recommended application(s)
Halogenated compounds
Recommended application(s)
Isomers
Recommended application(s)
Basic compounds
USP listing
L43
Phase
NUCLEODUR PFP
Carbon content
8.0 %
Surface chemistry
Pentafluorophenylpropyl (PFP) modification
Particle size
1.8 µm
Scope of delivery
1x analytical HPLC/UHPLC column, instruction leaflet, certificate of analysis
Method
HPLC/UHPLC, analytical
Type
EC UHPLC column (analytical)
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