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- EC HPLC column (analytical), NUCLEODUR PFP, 3 µm, 250x3 mm
This hydrophobic phase with pentafluorophenyl-propyl modification is based on high purity NUCLEODUR silica gel and gives an alternative selectivity in comparison to classical C18 modifications. Separation mechanism based on four retention mechanisms - polar interactions (H bonds), dipole-dipole interactions, π-π interactions and hydrophobic interactions. Suitable for LC/MS due to low bleeding characteristics.
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			Brand NUCLEODUR Pore size 110 Å Specific surface according to BET 340 m²/g Base material NUCLEODUR - high purity silica Particle type Fully porous particles (FPP) Particle shape Spherical Mode Reversed phase (RP) Endcapped Yes Recommended application(s) Antibiotics Recommended application(s) Phenols Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Recommended application(s) RP applications HPLC Recommended application(s) Reversed phase Recommended application(s) Pharmaceuticals Temperature stability (max) 60 °C Tmax (phosphate buffer) 40 °C Pressure stability (max) 600 bar Method HPLC, analytical Hardware Stainless steel Type EC HPLC column (analytical) Particle size 3 µm Pore volume 0.9 mL/g Eluent in HPLC column Acetonitrile – water Column inner diameter 3 mm Column length 250 mm Column volume 1.75 mL pH stability 1.0–9.0 Recommended application(s) Suitable for LC-MS Recommended application(s) Polar compounds Recommended application(s) Aromatic and unsaturated compounds Recommended application(s) Halogenated compounds Recommended application(s) Isomers Recommended application(s) Basic compounds USP listing L43 Phase NUCLEODUR PFP Carbon content 8.0 % Surface chemistry Pentafluorophenylpropyl (PFP) modification 
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