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- EC HPLC column (analytical), NUCLEODUR PFP, 5 µm, 50x3 mm
This hydrophobic phase with pentafluorophenyl-propyl modification is based on high purity NUCLEODUR silica gel and gives an alternative selectivity in comparison to classical C18 modifications. Separation mechanism based on four retention mechanisms - polar interactions (H bonds), dipole-dipole interactions, π-π interactions and hydrophobic interactions. Suitable for LC/MS due to low bleeding characteristics.
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Brand
NUCLEODUR
Pore size
110 Å
Specific surface according to BET
340 m²/g
Base material
NUCLEODUR - high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Mode
Reversed phase (RP)
Endcapped
Yes
Particle size
5 µm
Recommended application(s)
Antibiotics
Recommended application(s)
Phenols
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Recommended application(s)
RP applications HPLC
Recommended application(s)
Reversed phase
Recommended application(s)
Pharmaceuticals
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Column length
50 mm
Pressure stability (max)
600 bar
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
3 mm
Column volume
0.35 mL
pH stability
1.0–9.0
Recommended application(s)
Suitable for LC-MS
Recommended application(s)
Polar compounds
Recommended application(s)
Aromatic and unsaturated compounds
Recommended application(s)
Halogenated compounds
Recommended application(s)
Isomers
Recommended application(s)
Basic compounds
USP listing
L43
Phase
NUCLEODUR PFP
Carbon content
8.0 %
Surface chemistry
Pentafluorophenylpropyl (PFP) modification
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