EC HPLC column (analytical), NUCLEODUR PFP, 5 µm, 125x4.6 mm
 

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Macherey Nagel - Chromatography / HPLC column (analytical)

EC HPLC column (analytical), NUCLEODUR PFP, 5 µm, 125x4.6 mm

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SKU: 760457.46

EC HPLC column (analytical), NUCLEODUR PFP, 5 µm, 125x4.6 mm

This hydrophobic phase with pentafluorophenyl-propyl modification is based on high purity NUCLEODUR silica gel and gives an alternative selectivity in comparison to classical C18 modifications. Separation mechanism based on four retention mechanisms - polar interactions (H bonds), dipole-dipole interactions, π-π interactions and hydrophobic interactions. Suitable for LC/MS due to low bleeding characteristics.

  • Brand

    NUCLEODUR

    Pore size

    110 Å

    Specific surface according to BET

    340 m²/g

    Base material

    NUCLEODUR - high purity silica

    Particle type

    Fully porous particles (FPP)

    Particle shape

    Spherical

    Mode

    Reversed phase (RP)

    Endcapped

    Yes

    Particle size

    5 µm

    Recommended application(s)

    Antibiotics

    Recommended application(s)

    Phenols

    Scope of delivery

    1x analytical HPLC column, instruction leaflet, certificate of analysis

    Recommended application(s)

    RP applications HPLC

    Recommended application(s)

    Reversed phase

    Recommended application(s)

    Pharmaceuticals

    Temperature stability (max)

    60 °C

    Tmax (phosphate buffer)

    40 °C

    Pressure stability (max)

    600 bar

    Method

    HPLC, analytical

    Hardware

    Stainless steel

    Type

    EC HPLC column (analytical)

    Pore volume

    0.9 mL/g

    Eluent in HPLC column

    Acetonitrile – water

    Column inner diameter

    4.6 mm

    Column length

    125 mm

    Column volume

    2.06 mL

    pH stability

    1.0–9.0

    Recommended application(s)

    Suitable for LC-MS

    Recommended application(s)

    Polar compounds

    Recommended application(s)

    Aromatic and unsaturated compounds

    Recommended application(s)

    Halogenated compounds

    Recommended application(s)

    Isomers

    Recommended application(s)

    Basic compounds

    USP listing

    L43

    Phase

    NUCLEODUR PFP

    Carbon content

    8.0 %

    Surface chemistry

    Pentafluorophenylpropyl (PFP) modification

  • Macherey Nagel - Chromatography Catalogue