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- EC UHPLC column (analytical), NUCLEODUR PolarTec, 1.8 µm, 100x4.6 mm
Based on high-purity NUCLEODUR silica gel, a polar group was embedded in a hydrophobic C18 modification. As a result, this HPLC phase shows steric and an increased polar selectivity. This HPLC phase shows a good performance under high aqueous conditions. It is a hydrophobic phase with pronounced hydrophilic properties. Separation mechanism based on hydrophobic (van der Waals) and polar interactions. Suitable for LC/MS due to low bleeding characteristics.
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Brand
NUCLEODUR
Pore size
110 Å
Specific surface according to BET
340 m²/g
Base material
NUCLEODUR - high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Mode
Reversed phase (RP)
USP listing
L1
Endcapped
Yes
Recommended application(s)
Pesticides
Recommended application(s)
Phenols
Recommended application(s)
Amino acids
Recommended application(s)
Organic acids
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Pressure stability (max)
600 bar
Hardware
Stainless steel
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
4.6 mm
Column length
100 mm
Column volume
1.65 mL
Recommended application(s)
Water-soluble vitamins
pH stability
1.0–9.0
Recommended application(s)
Suitable for LC-MS
Recommended application(s)
Polar compounds
Carbon content
17.0 %
Recommended application(s)
Suitable for 100 % aqueous eluents
Recommended application(s)
RP applications UHPLC
Recommended application(s)
Nitrogen containing compounds
Recommended application(s)
Basic pharmaceuticals
USP listing
L60
Phase
NUCLEODUR PolarTec
Surface chemistry
Octadecyl phase (C18, ODS) modification
Surface chemistry
Embedded polar group (EPG, PEG)
Particle size
1.8 µm
Scope of delivery
1x analytical HPLC/UHPLC column, instruction leaflet, certificate of analysis
Method
HPLC/UHPLC, analytical
Type
EC UHPLC column (analytical)
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