EC HPLC column (analytical), NUCLEODUR PolarTec, 5 µm, 125x4.6 mm
 

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Macherey Nagel - Chromatography / HPLC column (analytical)

EC HPLC column (analytical), NUCLEODUR PolarTec, 5 µm, 125x4.6 mm

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SKU: 760487.46

EC HPLC column (analytical), NUCLEODUR PolarTec, 5 µm, 125x4.6 mm

Based on high-purity NUCLEODUR silica gel, a polar group was embedded in a hydrophobic C18 modification. As a result, this HPLC phase shows steric and an increased polar selectivity. This HPLC phase shows a good performance under high aqueous conditions. It is a hydrophobic phase with pronounced hydrophilic properties. Separation mechanism based on hydrophobic (van der Waals) and polar interactions. Suitable for LC/MS due to low bleeding characteristics.

  • Brand

    NUCLEODUR

    Pore size

    110 Å

    Specific surface according to BET

    340 m²/g

    Base material

    NUCLEODUR - high purity silica

    Particle type

    Fully porous particles (FPP)

    Particle shape

    Spherical

    Mode

    Reversed phase (RP)

    USP listing

    L1

    Endcapped

    Yes

    Particle size

    5 µm

    Recommended application(s)

    Pesticides

    Recommended application(s)

    Phenols

    Recommended application(s)

    Amino acids

    Recommended application(s)

    Organic acids

    Scope of delivery

    1x analytical HPLC column, instruction leaflet, certificate of analysis

    Recommended application(s)

    Reversed phase

    Temperature stability (max)

    60 °C

    Tmax (phosphate buffer)

    40 °C

    Pressure stability (max)

    600 bar

    Method

    HPLC, analytical

    Hardware

    Stainless steel

    Type

    EC HPLC column (analytical)

    Pore volume

    0.9 mL/g

    Eluent in HPLC column

    Acetonitrile – water

    Column inner diameter

    4.6 mm

    Column length

    125 mm

    Column volume

    2.06 mL

    Recommended application(s)

    Water-soluble vitamins

    pH stability

    1.0–9.0

    Recommended application(s)

    Suitable for LC-MS

    Recommended application(s)

    Polar compounds

    Carbon content

    17.0 %

    Recommended application(s)

    Suitable for 100 % aqueous eluents

    Recommended application(s)

    RP applications UHPLC

    Recommended application(s)

    Nitrogen containing compounds

    Recommended application(s)

    Basic pharmaceuticals

    USP listing

    L60

    Phase

    NUCLEODUR PolarTec

    Surface chemistry

    Octadecyl phase (C18, ODS) modification

    Surface chemistry

    Embedded polar group (EPG, PEG)

  • Macherey Nagel - Chromatography Catalogue