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- EC UHPLC column (analytical), NUCLEODUR HILIC, 1.8 µm, 50x2 mm
Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on high purity NUCLEODUR silica gel.
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			Brand NUCLEODUR Pore size 110 Å Specific surface according to BET 340 m²/g Base material NUCLEODUR - high purity silica Particle type Fully porous particles (FPP) Particle shape Spherical Endcapped No USP listing Not available or proprietary Recommended application(s) Amino acids Temperature stability (max) 60 °C Tmax (phosphate buffer) 40 °C Column length 50 mm Column inner diameter 2 mm Column volume 0.15 mL Hardware Stainless steel Pore volume 0.9 mL/g Eluent in HPLC column Acetonitrile – water Recommended application(s) Water-soluble vitamins Recommended application(s) Peptides Carbon content 7.0 % Recommended application(s) Highly polar analytes Recommended application(s) Hydrophilic compounds Recommended application(s) Organic polar acids and bases Recommended application(s) Polar natural compounds Recommended application(s) Nucleosides Recommended application(s) Oligonucleotides pH stability 2.0–8.5 Mode Hydrophilic Interaction Chromatography (HILIC) Phase NUCLEODUR HILIC Surface chemistry Ammonium – sulfonic acid (zwitterionic) modification Pressure stability (max) 900 bar Particle size 1.8 µm Scope of delivery 1x analytical HPLC/UHPLC column, instruction leaflet, certificate of analysis Method HPLC/UHPLC, analytical Type EC UHPLC column (analytical) Recommended application(s) HILIC applications UHPLC 
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