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- EC UHPLC column (analytical), NUCLEODUR HILIC, 1.8 µm, 100x3 mm
Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on high purity NUCLEODUR silica gel.
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Brand
NUCLEODUR
Pore size
110 Å
Specific surface according to BET
340 m²/g
Base material
NUCLEODUR - high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
USP listing
Not available or proprietary
Recommended application(s)
Amino acids
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Hardware
Stainless steel
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
3 mm
Column length
100 mm
Column volume
0.7 mL
Recommended application(s)
Water-soluble vitamins
Recommended application(s)
Peptides
Carbon content
7.0 %
Recommended application(s)
Highly polar analytes
Recommended application(s)
Hydrophilic compounds
Recommended application(s)
Organic polar acids and bases
Recommended application(s)
Polar natural compounds
Recommended application(s)
Nucleosides
Recommended application(s)
Oligonucleotides
pH stability
2.0–8.5
Mode
Hydrophilic Interaction Chromatography (HILIC)
Phase
NUCLEODUR HILIC
Surface chemistry
Ammonium – sulfonic acid (zwitterionic) modification
Particle size
1.8 µm
Pressure stability (max)
800 bar
Scope of delivery
1x analytical HPLC/UHPLC column, instruction leaflet, certificate of analysis
Method
HPLC/UHPLC, analytical
Type
EC UHPLC column (analytical)
Recommended application(s)
HILIC applications UHPLC
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