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- EC HPLC column (analytical), NUCLEODUR HILIC, 3 µm, 125x2 mm
Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on high purity NUCLEODUR silica gel.
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			Brand NUCLEODUR Pore size 110 Å Specific surface according to BET 340 m²/g Base material NUCLEODUR - high purity silica Particle type Fully porous particles (FPP) Particle shape Spherical Endcapped No USP listing Not available or proprietary Recommended application(s) Amino acids Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis Temperature stability (max) 60 °C Tmax (phosphate buffer) 40 °C Column inner diameter 2 mm Pressure stability (max) 600 bar Method HPLC, analytical Hardware Stainless steel Type EC HPLC column (analytical) Particle size 3 µm Pore volume 0.9 mL/g Eluent in HPLC column Acetonitrile – water Column length 125 mm Column volume 0.37 mL Recommended application(s) Water-soluble vitamins Recommended application(s) Peptides Carbon content 7.0 % Recommended application(s) HILIC applications HPLC Recommended application(s) Highly polar analytes Recommended application(s) Hydrophilic compounds Recommended application(s) Organic polar acids and bases Recommended application(s) Polar natural compounds Recommended application(s) Nucleosides Recommended application(s) Oligonucleotides pH stability 2.0–8.5 Mode Hydrophilic Interaction Chromatography (HILIC) Phase NUCLEODUR HILIC Surface chemistry Ammonium – sulfonic acid (zwitterionic) modification 
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