EC HPLC column (analytical), NUCLEODUR HILIC, 5 µm, 125x4.6 mm
 

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Macherey Nagel - Chromatography / HPLC column (analytical)

EC HPLC column (analytical), NUCLEODUR HILIC, 5 µm, 125x4.6 mm

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SKU: 760551.46

EC HPLC column (analytical), NUCLEODUR HILIC, 5 µm, 125x4.6 mm

Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on high purity NUCLEODUR silica gel.

  • Brand

    NUCLEODUR

    Pore size

    110 Å

    Specific surface according to BET

    340 m²/g

    Base material

    NUCLEODUR - high purity silica

    Particle type

    Fully porous particles (FPP)

    Particle shape

    Spherical

    Endcapped

    No

    Particle size

    5 µm

    USP listing

    Not available or proprietary

    Recommended application(s)

    Amino acids

    Scope of delivery

    1x analytical HPLC column, instruction leaflet, certificate of analysis

    Temperature stability (max)

    60 °C

    Tmax (phosphate buffer)

    40 °C

    Pressure stability (max)

    600 bar

    Method

    HPLC, analytical

    Hardware

    Stainless steel

    Type

    EC HPLC column (analytical)

    Pore volume

    0.9 mL/g

    Eluent in HPLC column

    Acetonitrile – water

    Column inner diameter

    4.6 mm

    Column length

    125 mm

    Column volume

    2.06 mL

    Recommended application(s)

    Water-soluble vitamins

    Recommended application(s)

    Peptides

    Carbon content

    7.0 %

    Recommended application(s)

    HILIC applications HPLC

    Recommended application(s)

    Highly polar analytes

    Recommended application(s)

    Hydrophilic compounds

    Recommended application(s)

    Organic polar acids and bases

    Recommended application(s)

    Polar natural compounds

    Recommended application(s)

    Nucleosides

    Recommended application(s)

    Oligonucleotides

    pH stability

    2.0–8.5

    Mode

    Hydrophilic Interaction Chromatography (HILIC)

    Phase

    NUCLEODUR HILIC

    Surface chemistry

    Ammonium – sulfonic acid (zwitterionic) modification

  • Macherey Nagel - Chromatography Catalogue