EC HPLC column (analytical), NUCLEODUR HILIC, 5 µm, 100x4.6 mm
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Brand
NUCLEODUR
Pore size
110 Å
Specific surface according to BET
340 m²/g
Base material
NUCLEODUR - high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
Particle size
5 µm
USP listing
Not available or proprietary
Recommended application(s)
Amino acids
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Pressure stability (max)
600 bar
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
4.6 mm
Column length
100 mm
Column volume
1.65 mL
Recommended application(s)
Water-soluble vitamins
Recommended application(s)
Peptides
Carbon content
7.0 %
Recommended application(s)
HILIC applications HPLC
Recommended application(s)
Highly polar analytes
Recommended application(s)
Hydrophilic compounds
Recommended application(s)
Organic polar acids and bases
Recommended application(s)
Polar natural compounds
Recommended application(s)
Nucleosides
Recommended application(s)
Oligonucleotides
pH stability
2.0–8.5
Mode
Hydrophilic Interaction Chromatography (HILIC)
Phase
NUCLEODUR HILIC
Surface chemistry
Ammonium – sulfonic acid (zwitterionic) modification