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- EC HPLC column (analytical), NUCLEODUR Phenyl-Hexyl, 5 µm, 50x4 mm
This hydrophobic phase is based on high purity NUCLEODUR silica gel and modified with phenylhexyl. This RP phase gives an alternative selectivity in comparison to classical C18 modifications. Separation principle based on two retention mechanisms - π-π interactions and hydrophobic interactions. Due to low bleeding characteristics this HPLC phase is suitable for LC/MS.
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Brand
NUCLEODUR
Pore size
110 Å
Specific surface according to BET
340 m²/g
Base material
NUCLEODUR - high purity silica
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Mode
Reversed phase (RP)
Endcapped
Yes
Particle size
5 µm
Recommended application(s)
Steroids
Recommended application(s)
Antibiotics
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Recommended application(s)
RP applications HPLC
Recommended application(s)
Reversed phase
Recommended application(s)
Pharmaceuticals
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Column length
50 mm
Pressure stability (max)
600 bar
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Pore volume
0.9 mL/g
Eluent in HPLC column
Acetonitrile – water
Column inner diameter
4 mm
Column volume
0.62 mL
Recommended application(s)
Suitable for LC-MS
Recommended application(s)
Polar compounds
USP listing
L11
pH stability
1.0–10.0
Recommended application(s)
Aromatic and unsaturated compounds
Phase
NUCLEODUR Phenyl-Hexyl
Carbon content
10.0 %
Surface chemistry
Phenylhexyl modification
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