EC HPLC column (analytical), NUCLEODUR Phenyl-Hexyl, 5 µm, 125x4.6 mm

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SKU: 760587.46

EC HPLC column (analytical), NUCLEODUR Phenyl-Hexyl, 5 µm, 125x4.6 mm

This hydrophobic phase is based on high purity NUCLEODUR silica gel and modified with phenylhexyl. This RP phase gives an alternative selectivity in comparison to classical C18 modifications. Separation principle based on two retention mechanisms - π-π interactions and hydrophobic interactions. Due to low bleeding characteristics this HPLC phase is suitable for LC/MS.
  • Brand

    NUCLEODUR

    Pore size

    110 Å

    Specific surface according to BET

    340 m²/g

    Base material

    NUCLEODUR - high purity silica

    Particle type

    Fully porous particles (FPP)

    Particle shape

    Spherical

    Mode

    Reversed phase (RP)

    Endcapped

    Yes

    Particle size

    5 µm

    Recommended application(s)

    Steroids

    Recommended application(s)

    Antibiotics

    Scope of delivery

    1x analytical HPLC column, instruction leaflet, certificate of analysis

    Recommended application(s)

    RP applications HPLC

    Recommended application(s)

    Reversed phase

    Recommended application(s)

    Pharmaceuticals

    Temperature stability (max)

    60 °C

    Tmax (phosphate buffer)

    40 °C

    Pressure stability (max)

    600 bar

    Method

    HPLC, analytical

    Hardware

    Stainless steel

    Type

    EC HPLC column (analytical)

    Pore volume

    0.9 mL/g

    Eluent in HPLC column

    Acetonitrile – water

    Column inner diameter

    4.6 mm

    Column length

    125 mm

    Column volume

    2.06 mL

    Recommended application(s)

    Suitable for LC-MS

    Recommended application(s)

    Polar compounds

    USP listing

    L11

    pH stability

    1.0–10.0

    Recommended application(s)

    Aromatic and unsaturated compounds

    Phase

    NUCLEODUR Phenyl-Hexyl

    Carbon content

    10.0 %

    Surface chemistry

    Phenylhexyl modification

  • Macherey Nagel - Chromatography Catalogue