EC HPLC column (analytical), NUCLEOSHELL RP 18plus, 2.7 µm, 50x2 mm
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Particle shape
Spherical
Mode
Reversed phase (RP)
USP listing
L1
Endcapped
Yes
Pore size
90 Å
Recommended application(s)
Antibiotics
Recommended application(s)
Organic acids
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Recommended application(s)
RP applications HPLC
Recommended application(s)
Reversed phase
Recommended application(s)
Pharmaceuticals
Temperature stability (max)
60 °C
Tmax (phosphate buffer)
40 °C
Column length
50 mm
Column inner diameter
2 mm
Pressure stability (max)
600 bar
Column volume
0.15 mL
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Eluent in HPLC column
Acetonitrile – water
Recommended application(s)
Especially for polar compounds
Recommended application(s)
Water-soluble vitamins
pH stability
2.0–9.0
Brand
NUCLEOSHELL
Pore volume
Not available or proprietary
Base material
NUCLEOSHELL - core-shell silica
Particle type
Core-shell, superficially porous particles (SPP)
Phase
NUCLEOSHELL RP 18plus
Surface chemistry
Monomeric octadecyl (C18, RP18, ODS) modification
Surface chemistry
Polar selectivity
Particle size
2.7 µm
Specific surface according to BET
130 m²/g
Carbon content
5.7 %
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Macherey Nagel - Chromatography Catalogue
PFAS analysis performed on MACHEREY-NAGEL products according to official methods like EPA and DIN
EPA 533: The enrichment of PFAS using the SPE column CHROMABOND HR-XAW and subsequent chromatographic separation on a NUCLEODUR PFAS analytical HPLC column: PFAS analysis according to EPA 533
EPA 537.1: The enrichment of PFAS using the SPE column CHROMABOND HR-X and subsequent chromatographic separation on a NUCLEODUR PFAS analytical HPLC column: PFAS analysis according to EPA 537.1
Further Application Notes related to PFAS analysis:
Download: Application Note (PDF) - PFAS analysis according to FDA method C-010.02
Download: Application Note (PDF) - PFAS analysis according to EPA 533 and 537.1
Download: Application Note (PDF) - PFAS analysis according to ISO 21675:2019 and to DIN 38407‑42