QC INSTR PERF CHK STD 125mL

Instrument Performance Check Solution (IPC) -- The Initial Calibration Check Standard is to be evaluated as the instrument performance check solution in order to confirm proper instrument performance. Proper chromatographic performance must be demonstrated by calculating the Peak Gaussian Factor (PGF), which is a means to measure peak symmetry and monitoring retention time drift in the surrogate peak over time.

The retention time for the surrogate in the IPC must be closely monitored on each day of analysis and throughout the lifetime of the analytical column. Small variations in retention time can be anticipated when a new solution of eluent is prepared but if shifts of more than 2% are observed in the surrogate retention time, some type of instrument problem is present. Potential problems include improperly prepared eluent, erroneous method parameters programmed such as flow rate or some other system problem. The chromatographic profile (elution order) of the target anions following an ion chromatographic analysis should closely replicate the profile displayed in the test chromatogram that was shipped when the column was purchased. As a column ages, it is normal to see a gradual shift and shortening of retention times, but if after several years of use, extensive use over less than a year, or use with harsh samples, this retention time has noticeably shifted to any less than 80% of the original recorded value, the column may require cleaning or replacement. Particularly if resolution problems are beginning to become common between previously resolved peaks. A laboratory must retain a historic record of retention times for the surrogate and all the target anions to provide evidence of an analytical columns vitality.