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- EC HPLC column (analytical), NUCLEODEX BETA-OH, 5 µm, 200x4 mm
Cyclodextrin-modified silica phases for HPLC enantiomer separation. Separation based on hydrogen bonds and dipole interactions between functional groups of the analyte and hydroxyl groups of the cyclodextrin. For compounds, which require free hydroxyl groups for enantioselective interactions.
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Recommended application(s)
HPLC
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Endcapped
No
Mode
Special application or diverse
Pore size
100 Å
Particle size
5 µm
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Method
HPLC, analytical
Hardware
Stainless steel
Type
EC HPLC column (analytical)
Column inner diameter
4 mm
Column volume
2.5 mL
Carbon content
Not available or proprietary
Pressure stability (max)
400 bar
Pore volume
1 mL/g
Specific surface according to BET
350 m²/g
Base material
NUCLEOSIL - the standard silica
Brand
NUCLEODEX
Recommended application(s)
Chiral, enantiomers
Recommended application(s)
Enantiomers
Recommended application(s)
Chlorthalidone
Recommended application(s)
Compounds, which require free hydroxyl groups for enantioselective interactions
Temperature stability (max)
50 °C
Column length
200 mm
pH stability
3.0–8.0
USP listing
L45
Phase
NUCLEODEX BETA-OH
Surface chemistry
β-cyclodextrin (chiral selector) modification
Eluent in HPLC column
CH₃OH (methanol) – 0.1 % TEAA (triethylammonium acetate), pH 4 (55/45, v/v)
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